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seqman ii software packages  (DNASTAR)


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    DNASTAR seqman ii software packages
    Seqman Ii Software Packages, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 10689 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ii+software+package/Lasergene/10__1016_slash_j__heha__2026__100173-103-5-10
    Average 99 stars, based on 10689 article reviews
    seqman ii software packages - by Bioz Stars, 2026-09
    99/100 stars

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    Software:

    Article Title: Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-?-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Norway
    Article Snippet: Bidirectional sequencing was performed using a BigDye v. 3.1 cycle sequencing kit and a model 3100 genetic analyzer (Applied Biosystems). .. Editing and alignment of DNA sequences were performed using the SeqMan II software package (DNAStar, Inc., Madison, WI). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Amplicon Primer sequence (5′ to 3′) Size of amplicon (bp) Annealing temp (°C) Reference or source 16S rRNA gene AGA GTT TGA TCM TGG CTC AG ∼1,500 55 27a ACG GHT ACC TTG TTA CGA CTT bla TEM ATG AGT ATT CAA CAT TTC CG 858 50 This study CCA ATG CTT AAT CAG TGA GG bla SHV ATG CGT TAT ATT CGC CTG TG 862 58 This study AGC GTT GCC AGT GCT CGA TC bla CTX -M SCS ATG TGC AGY ACC AGT AA 585 58 This study ACC AGA AYV AGC GGB GC Open in a separate window PCR primers used in this study .. Analytic isoelectric focusing (IEF) of sonicated crude cell extracts was performed in precast Ampholine PAGplate polyacrylamide gels with a pH range of 3.5 to 9.5 (GE Healthcare, St. Giles, United Kingdom) using a Multiphor II apparatus (GE Healthcare). β-Lactamase activity was detected with nitrocefin (0.5 g/liter).

    Article Title: The Origins of 168, W23, and Other Bacillus subtilis Legacy Strains
    Article Snippet: .. Contiguous sequences were assembled with the SeqMan II software package (DNAStar, Inc.). ..

    Article Title: Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-?-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Norway
    Article Snippet: Bidirectional sequencing was performed using a BigDye v. 3.1 cycle sequencing kit and a model 3100 genetic analyzer (Applied Biosystems). .. Editing and alignment of DNA sequences were performed using the SeqMan II software package (DNAStar, Inc., Madison, WI). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Amplicon Primer sequence (5′ to 3′) Size of amplicon (bp) Annealing temp (°C) Reference or source 16S rRNA gene AGA GTT TGA TCM TGG CTC AG ∼1,500 55 27a ACG GHT ACC TTG TTA CGA CTT bla TEM ATG AGT ATT CAA CAT TTC CG 858 50 This study CCA ATG CTT AAT CAG TGA GG bla SHV ATG CGT TAT ATT CGC CTG TG 862 58 This study AGC GTT GCC AGT GCT CGA TC bla CTX -M SCS ATG TGC AGY ACC AGT AA 585 58 This study ACC AGA AYV AGC GGB GC Open in a separate window PCR primers used in this study Isoelectric focusing of β-lactamases. .. Analytic isoelectric focusing (IEF) of sonicated crude cell extracts was performed in precast Ampholine PAGplate polyacrylamide gels with a pH range of 3.5 to 9.5 (GE Healthcare, St. Giles, United Kingdom) using a Multiphor II apparatus (GE Healthcare). β-Lactamase activity was detected with nitrocefin (0.5 g/liter).

    Article Title: A novel simian immunodeficiency virus isolated from a Schmidt's guenon (Cercopithecus ascanius schmidti).
    Article Snippet: PCR products were isolated from agarose gel using the QIAquick Gel Extraction kit (Qiagen) and sequenced directly using the ABI PRISM BigDye Terminators V3.0 Cycle Sequencing kit on an ABI PRISM 3100 Genetic Analyser (Applied Biosystems). .. Sequence analysis was performed using the Seqman II software package (DNASTAR). ..

    Article Title: Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-β-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Norway
    Article Snippet: Bidirectional sequencing was performed using a BigDye v. 3.1 cycle sequencing kit and a model 3100 genetic analyzer (Applied Biosystems). .. Editing and alignment of DNA sequences were performed using the SeqMan II software package (DNAStar, Inc., Madison, WI). ..

    Article Title: Genomic Analysis in the sting-2 Quantitative Trait Locus for Defensive Behavior in the Honey Bee, Apis mellifera
    Article Snippet: Plasmids cloned from all libraries were sequenced from both ends of the inserts with standard M13 forward and reverse primers using ABI Big Dye Terminator v2, and reactions were analyzed on the ABI Prism 3700 DNA Analyzer. .. Sequencing data was evaluated, trimmed, and assembled using SEQMAN II software package (DNASTAR Inc.; Swindell and Plasterer 1997 ). ..

    Article Title: Genomic analysis of a 1 Mb region near the telomere of Hessian fly chromosome X2 and avirulence gene vH13
    Article Snippet: Plasmids cloned from all libraries were sequenced from both ends of the inserts with standard M13 forward and reverse primers using ABI Big Dye Terminator v3, and reactions were analyzed on the ABI Prism ® 3700 DNA Analyzer. .. Sequencing data was evaluated, trimmed and assembled using SEQMAN II software package (DNASTAR Inc.) [ ]. ..

    Amplification:

    Article Title: Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-?-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Norway
    Article Snippet: Bidirectional sequencing was performed using a BigDye v. 3.1 cycle sequencing kit and a model 3100 genetic analyzer (Applied Biosystems). .. Editing and alignment of DNA sequences were performed using the SeqMan II software package (DNAStar, Inc., Madison, WI). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Amplicon Primer sequence (5′ to 3′) Size of amplicon (bp) Annealing temp (°C) Reference or source 16S rRNA gene AGA GTT TGA TCM TGG CTC AG ∼1,500 55 27a ACG GHT ACC TTG TTA CGA CTT bla TEM ATG AGT ATT CAA CAT TTC CG 858 50 This study CCA ATG CTT AAT CAG TGA GG bla SHV ATG CGT TAT ATT CGC CTG TG 862 58 This study AGC GTT GCC AGT GCT CGA TC bla CTX -M SCS ATG TGC AGY ACC AGT AA 585 58 This study ACC AGA AYV AGC GGB GC Open in a separate window PCR primers used in this study .. Analytic isoelectric focusing (IEF) of sonicated crude cell extracts was performed in precast Ampholine PAGplate polyacrylamide gels with a pH range of 3.5 to 9.5 (GE Healthcare, St. Giles, United Kingdom) using a Multiphor II apparatus (GE Healthcare). β-Lactamase activity was detected with nitrocefin (0.5 g/liter).

    Article Title: Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-?-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Norway
    Article Snippet: Bidirectional sequencing was performed using a BigDye v. 3.1 cycle sequencing kit and a model 3100 genetic analyzer (Applied Biosystems). .. Editing and alignment of DNA sequences were performed using the SeqMan II software package (DNAStar, Inc., Madison, WI). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Amplicon Primer sequence (5′ to 3′) Size of amplicon (bp) Annealing temp (°C) Reference or source 16S rRNA gene AGA GTT TGA TCM TGG CTC AG ∼1,500 55 27a ACG GHT ACC TTG TTA CGA CTT bla TEM ATG AGT ATT CAA CAT TTC CG 858 50 This study CCA ATG CTT AAT CAG TGA GG bla SHV ATG CGT TAT ATT CGC CTG TG 862 58 This study AGC GTT GCC AGT GCT CGA TC bla CTX -M SCS ATG TGC AGY ACC AGT AA 585 58 This study ACC AGA AYV AGC GGB GC Open in a separate window PCR primers used in this study Isoelectric focusing of β-lactamases. .. Analytic isoelectric focusing (IEF) of sonicated crude cell extracts was performed in precast Ampholine PAGplate polyacrylamide gels with a pH range of 3.5 to 9.5 (GE Healthcare, St. Giles, United Kingdom) using a Multiphor II apparatus (GE Healthcare). β-Lactamase activity was detected with nitrocefin (0.5 g/liter).

    Sequencing:

    Article Title: Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-?-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Norway
    Article Snippet: Bidirectional sequencing was performed using a BigDye v. 3.1 cycle sequencing kit and a model 3100 genetic analyzer (Applied Biosystems). .. Editing and alignment of DNA sequences were performed using the SeqMan II software package (DNAStar, Inc., Madison, WI). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Amplicon Primer sequence (5′ to 3′) Size of amplicon (bp) Annealing temp (°C) Reference or source 16S rRNA gene AGA GTT TGA TCM TGG CTC AG ∼1,500 55 27a ACG GHT ACC TTG TTA CGA CTT bla TEM ATG AGT ATT CAA CAT TTC CG 858 50 This study CCA ATG CTT AAT CAG TGA GG bla SHV ATG CGT TAT ATT CGC CTG TG 862 58 This study AGC GTT GCC AGT GCT CGA TC bla CTX -M SCS ATG TGC AGY ACC AGT AA 585 58 This study ACC AGA AYV AGC GGB GC Open in a separate window PCR primers used in this study .. Analytic isoelectric focusing (IEF) of sonicated crude cell extracts was performed in precast Ampholine PAGplate polyacrylamide gels with a pH range of 3.5 to 9.5 (GE Healthcare, St. Giles, United Kingdom) using a Multiphor II apparatus (GE Healthcare). β-Lactamase activity was detected with nitrocefin (0.5 g/liter).

    Article Title: Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-?-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Norway
    Article Snippet: Bidirectional sequencing was performed using a BigDye v. 3.1 cycle sequencing kit and a model 3100 genetic analyzer (Applied Biosystems). .. Editing and alignment of DNA sequences were performed using the SeqMan II software package (DNAStar, Inc., Madison, WI). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Amplicon Primer sequence (5′ to 3′) Size of amplicon (bp) Annealing temp (°C) Reference or source 16S rRNA gene AGA GTT TGA TCM TGG CTC AG ∼1,500 55 27a ACG GHT ACC TTG TTA CGA CTT bla TEM ATG AGT ATT CAA CAT TTC CG 858 50 This study CCA ATG CTT AAT CAG TGA GG bla SHV ATG CGT TAT ATT CGC CTG TG 862 58 This study AGC GTT GCC AGT GCT CGA TC bla CTX -M SCS ATG TGC AGY ACC AGT AA 585 58 This study ACC AGA AYV AGC GGB GC Open in a separate window PCR primers used in this study Isoelectric focusing of β-lactamases. .. Analytic isoelectric focusing (IEF) of sonicated crude cell extracts was performed in precast Ampholine PAGplate polyacrylamide gels with a pH range of 3.5 to 9.5 (GE Healthcare, St. Giles, United Kingdom) using a Multiphor II apparatus (GE Healthcare). β-Lactamase activity was detected with nitrocefin (0.5 g/liter).

    Article Title: A novel simian immunodeficiency virus isolated from a Schmidt's guenon (Cercopithecus ascanius schmidti).
    Article Snippet: PCR products were isolated from agarose gel using the QIAquick Gel Extraction kit (Qiagen) and sequenced directly using the ABI PRISM BigDye Terminators V3.0 Cycle Sequencing kit on an ABI PRISM 3100 Genetic Analyser (Applied Biosystems). .. Sequence analysis was performed using the Seqman II software package (DNASTAR). ..

    Article Title: Genomic Analysis in the sting-2 Quantitative Trait Locus for Defensive Behavior in the Honey Bee, Apis mellifera
    Article Snippet: Plasmids cloned from all libraries were sequenced from both ends of the inserts with standard M13 forward and reverse primers using ABI Big Dye Terminator v2, and reactions were analyzed on the ABI Prism 3700 DNA Analyzer. .. Sequencing data was evaluated, trimmed, and assembled using SEQMAN II software package (DNASTAR Inc.; Swindell and Plasterer 1997 ). ..

    Article Title: Health effects of green tea catechins in overweight and obese men: a randomised controlled cross-over trial
    Article Snippet: Then 10 ng of each purified PCR fragment were sequenced using an ABI PRISM dGTP BigDye terminator cycle sequencing kit (Applied Biosystems, Carlsbad, CA, USA); unincorporated fluorescent nucleotides were removed using a CleanSeq SPRI magnetic clean up kit (Beckman Coulter Genomics (formerly Agencourt Biosciences), Danvers, MA, USA). .. Sequencing reactions were separated on an ABI 3730 DNA sequencer and data analysed using the DNASTAR Seqman II software package (Lasergenew; DNASTAR, Inc., Madison, WI, USA). ..

    Article Title: Genomic analysis of a 1 Mb region near the telomere of Hessian fly chromosome X2 and avirulence gene vH13
    Article Snippet: Plasmids cloned from all libraries were sequenced from both ends of the inserts with standard M13 forward and reverse primers using ABI Big Dye Terminator v3, and reactions were analyzed on the ABI Prism ® 3700 DNA Analyzer. .. Sequencing data was evaluated, trimmed and assembled using SEQMAN II software package (DNASTAR Inc.) [ ]. ..

    Transmission Electron Microscopy:

    Article Title: Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-?-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Norway
    Article Snippet: Bidirectional sequencing was performed using a BigDye v. 3.1 cycle sequencing kit and a model 3100 genetic analyzer (Applied Biosystems). .. Editing and alignment of DNA sequences were performed using the SeqMan II software package (DNAStar, Inc., Madison, WI). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Amplicon Primer sequence (5′ to 3′) Size of amplicon (bp) Annealing temp (°C) Reference or source 16S rRNA gene AGA GTT TGA TCM TGG CTC AG ∼1,500 55 27a ACG GHT ACC TTG TTA CGA CTT bla TEM ATG AGT ATT CAA CAT TTC CG 858 50 This study CCA ATG CTT AAT CAG TGA GG bla SHV ATG CGT TAT ATT CGC CTG TG 862 58 This study AGC GTT GCC AGT GCT CGA TC bla CTX -M SCS ATG TGC AGY ACC AGT AA 585 58 This study ACC AGA AYV AGC GGB GC Open in a separate window PCR primers used in this study .. Analytic isoelectric focusing (IEF) of sonicated crude cell extracts was performed in precast Ampholine PAGplate polyacrylamide gels with a pH range of 3.5 to 9.5 (GE Healthcare, St. Giles, United Kingdom) using a Multiphor II apparatus (GE Healthcare). β-Lactamase activity was detected with nitrocefin (0.5 g/liter).

    Article Title: Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-?-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Norway
    Article Snippet: Bidirectional sequencing was performed using a BigDye v. 3.1 cycle sequencing kit and a model 3100 genetic analyzer (Applied Biosystems). .. Editing and alignment of DNA sequences were performed using the SeqMan II software package (DNAStar, Inc., Madison, WI). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Amplicon Primer sequence (5′ to 3′) Size of amplicon (bp) Annealing temp (°C) Reference or source 16S rRNA gene AGA GTT TGA TCM TGG CTC AG ∼1,500 55 27a ACG GHT ACC TTG TTA CGA CTT bla TEM ATG AGT ATT CAA CAT TTC CG 858 50 This study CCA ATG CTT AAT CAG TGA GG bla SHV ATG CGT TAT ATT CGC CTG TG 862 58 This study AGC GTT GCC AGT GCT CGA TC bla CTX -M SCS ATG TGC AGY ACC AGT AA 585 58 This study ACC AGA AYV AGC GGB GC Open in a separate window PCR primers used in this study Isoelectric focusing of β-lactamases. .. Analytic isoelectric focusing (IEF) of sonicated crude cell extracts was performed in precast Ampholine PAGplate polyacrylamide gels with a pH range of 3.5 to 9.5 (GE Healthcare, St. Giles, United Kingdom) using a Multiphor II apparatus (GE Healthcare). β-Lactamase activity was detected with nitrocefin (0.5 g/liter).

    Polymerase Chain Reaction:

    Article Title: Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-?-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Norway
    Article Snippet: Bidirectional sequencing was performed using a BigDye v. 3.1 cycle sequencing kit and a model 3100 genetic analyzer (Applied Biosystems). .. Editing and alignment of DNA sequences were performed using the SeqMan II software package (DNAStar, Inc., Madison, WI). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Amplicon Primer sequence (5′ to 3′) Size of amplicon (bp) Annealing temp (°C) Reference or source 16S rRNA gene AGA GTT TGA TCM TGG CTC AG ∼1,500 55 27a ACG GHT ACC TTG TTA CGA CTT bla TEM ATG AGT ATT CAA CAT TTC CG 858 50 This study CCA ATG CTT AAT CAG TGA GG bla SHV ATG CGT TAT ATT CGC CTG TG 862 58 This study AGC GTT GCC AGT GCT CGA TC bla CTX -M SCS ATG TGC AGY ACC AGT AA 585 58 This study ACC AGA AYV AGC GGB GC Open in a separate window PCR primers used in this study .. Analytic isoelectric focusing (IEF) of sonicated crude cell extracts was performed in precast Ampholine PAGplate polyacrylamide gels with a pH range of 3.5 to 9.5 (GE Healthcare, St. Giles, United Kingdom) using a Multiphor II apparatus (GE Healthcare). β-Lactamase activity was detected with nitrocefin (0.5 g/liter).

    Article Title: Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-?-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Norway
    Article Snippet: Bidirectional sequencing was performed using a BigDye v. 3.1 cycle sequencing kit and a model 3100 genetic analyzer (Applied Biosystems). .. Editing and alignment of DNA sequences were performed using the SeqMan II software package (DNAStar, Inc., Madison, WI). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Amplicon Primer sequence (5′ to 3′) Size of amplicon (bp) Annealing temp (°C) Reference or source 16S rRNA gene AGA GTT TGA TCM TGG CTC AG ∼1,500 55 27a ACG GHT ACC TTG TTA CGA CTT bla TEM ATG AGT ATT CAA CAT TTC CG 858 50 This study CCA ATG CTT AAT CAG TGA GG bla SHV ATG CGT TAT ATT CGC CTG TG 862 58 This study AGC GTT GCC AGT GCT CGA TC bla CTX -M SCS ATG TGC AGY ACC AGT AA 585 58 This study ACC AGA AYV AGC GGB GC Open in a separate window PCR primers used in this study Isoelectric focusing of β-lactamases. .. Analytic isoelectric focusing (IEF) of sonicated crude cell extracts was performed in precast Ampholine PAGplate polyacrylamide gels with a pH range of 3.5 to 9.5 (GE Healthcare, St. Giles, United Kingdom) using a Multiphor II apparatus (GE Healthcare). β-Lactamase activity was detected with nitrocefin (0.5 g/liter).



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